Role of Restriction Enzymes in Gene Cloning

Role of Restriction Enzymes in Gene Cloning

Restriction enzymes protect bacteria from invading bacteriophages in the natural world by slicing through foreign DNA. This DNA slicing is accomplished by the recognition of certain sequences at specific locations called recognition sites. Some restriction enzymes cut the two complementary DNA strands at their recognition sites, leaving one nucleotide overhang that can be joined with…